Plasma-Activated Water Modulates Root Hair Cell Density via Root Developmental Genes in Arabidopsis thaliana L.

Low-temperature atmospheric pressure plasma technology has been used in agriculture and plant science by direct indirect treatment of bio-samples. However, the cellular molecular mechanisms affected plasma-activated water (PAW) are largely unexplored. In this study, PAW generated from a surface dielectric barrier discharge (SDBD) device was for development. Physicochemical analysis performed to confirm properties that correlated with time. Arabidopsis thaliana L. utilized study effect early developmental stage. The samples denoted as PAW5 time minutes (min), PAW7 min, PAW12 PAW19 min PAW40 Seedlings grown PAW5, had increased root lengths while were decreased PAW40. level observation, specifically hair numbers per unit but suppressed length PAW, indicating mainly modulates cell density root. Furthermore, we found at maximal observed conditions positively regulated developmental-related genes including COBRA-LIKE9 (COBL9), XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE9 (XTH9), XTH17, AUXIN1 (AUX1) LIKE-AUXIN (LAX3).